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Neuronal Cell Death in Hippocampus Induced by Homocysteic Acid in Immature Rats by Miloš Langmeier; Jaroslava Folbergrová; Renata Haugvicová; Jaroslav Pokorný; Pavel Mareš is a Medicine article available to read on EtoBox.

What is Neuronal Cell Death in Hippocampus Induced by Homocysteic Acid in Immature Rats about?

**Summary:** **Purpose:** To examine the morphologic alterations in the cerebral cortex and hippocampus of immature rats 6 days after the generalized clonic–tonic seizures induced by homocysteic acid (HCA). **Methods:** Seizures were induced by bilateral intracerebroventricular infusion of HCA (600 nmol per each side) in 12‐day‐old rats. After 6 days, rat pups were transcardially perfused under deep ether anesthesia with heparinized normal saline and subsequently with the fixation solution (4% paraformaldehyde in phosphate buffer, pH 7.4, for light microscopy) or with Karnovsky's solution (4% paraformaldehyde and 2% glutaraldehyde in phosphate buffer, pH 7.4, for electron microscopic analysis). Nissl stain and the DNA‐specific dye bis‐benzimide (Hoechst 33342) were used. **Results:** No pathologic changes were found in the cerebral cortex, whereas serious alterations occurred in the hippocampus. A total loss of CA3 pyramidal cells was observed, with marked changes in the CA1 region and dentate gyrus. A prominent glial reaction was seen in many regions of the hippocampal formation. A slight dilatation of the cerebral ventricles was noticed in some experimental as well as control an

Who reads Neuronal Cell Death in Hippocampus Induced by Homocysteic Acid in Immature Rats?

Author

Miloš Langmeier; Jaroslava Folbergrová; Renata Haugvicová; Jaroslav Pokorný; Pavel Mareš

Publisher

Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0013-9580)

Published

2003

Language

EN

Field

Medicine (Health Sciences)