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Synergistic Effects of Hepatocyte Growth Factor on Human Cord Blood CD34 ^+^ Progenitor Cells are the Result of c‐met Receptor Expression by Julie P. Goff; Donna S. Shields; Bryon E. Petersen; Valerie F. Zajac; George K. Michalopoulos; Dr. Joel S. Greenberger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Hepatocyte growth factor (HGF) is a pleiotropic growth factor which, in addition to its mitogenic potency for primary hepatocytes, also has a role in the regulation of cell motility, cell growth and morphogenesis. In the present study, we show that c-met, the high-affinity receptor for HGF, is expressed on human cord blood (CB) CD34+ progenitor cells and CD34+Thy-1+ Lin-(lin-) cells. We have investigated the capacity of HGF to synergize with other growth factors to induce colony formation by CB CD34+ progenitor cells. CD34+ cells were cultured in semisolid medium containing serum with increasing concentrations of GM-CSF, G-CSF, macrophage colony-stimulating factor (M-CSF), stem cell factor (SCF), interleukin 3 (IL-3) and IL-11 alone or in combination with HGF. HGF acted as a potent synergist and enhanced, up to fourfold, colony formation induced by GM-CSF, G-CSF or M-CSF. HGF in combination with SCF, IL-3 or IL-11 did not induce proliferation of colony forming units-granulocyte macrophage (CFU-GM) above control levels. In serum-deprived cultures, HGF was only detectably synergistic with IL-11, and all other culture combinations showed no proliferation. To determine whether the stim

Who reads Synergistic Effects of Hepatocyte Growth Factor on Human Cord Blood CD34 ^+^ Progenitor Cells are the Result of c‐met Receptor Expression?

Author

Julie P. Goff; Donna S. Shields; Bryon E. Petersen; Valerie F. Zajac; George K. Michalopoulos; Dr. Joel S. Greenberger

Publisher

Oxford University Press (OUP)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)