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Can I read Crystallization and preliminary X-ray crystallographic analysis of DFPase from Loligo vulgaris on EtoBox?
Crystallization and preliminary X-ray crystallographic analysis of DFPase from Loligo vulgaris by Eileen I. Scharff; Christian Lücke; Günter Fritzsch; Juergen Koepke; Judith Hartleib; Stefan Dierl; Heinz Rüterjans is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Crystallization and preliminary X-ray crystallographic analysis of DFPase from Loligo vulgaris about?
'Squid-type' diisopropylfluorophosphatases (DFPases), a subclass of the phosphotriesterases, are enzymes capable of hydrolysing organophosphorus nerve agents. To date, no three-dimensional structure of a 'squid-type' DFPase is known. Here, the crystallization of the DFPase originally isolated from head ganglion of the squid Loligo vulgaris is reported. The protein has been heterologously expressed in Escherichia coli, purified to homogeneity and subsequently crystallized. The protein crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 43.1, b = 82.1, c = 86.6 A and one monomer per asymmetric unit. Under cryoconditions (120 K) the crystals diffracted beyond 2.0 A using a Cu rotating-anode X-ray generator.
Who reads Crystallization and preliminary X-ray crystallographic analysis of DFPase from Loligo vulgaris?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Eileen I. Scharff; Christian Lücke; Günter Fritzsch; Juergen Koepke; Judith Hartleib; Stefan Dierl; Heinz Rüterjans
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)