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Engineering Customized TALENs Using the Platinum Gate TALEN Kit by Kühn, Ralf; Wurst, Wolfgang; Wefers, Benedikt is a scholarly article available to read on EtoBox.

What is Engineering Customized TALENs Using the Platinum Gate TALEN Kit about?

This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field

Author

Kühn, Ralf; Wurst, Wolfgang; Wefers, Benedikt

Publisher

Springer New York : Imprint : Humana Press

Published

2016

Language

EN

ISBN

9781493929313