Opening book details…
Can I read Somatic embryogenesis in two Gossypium hirsutum genotypes on semi-solid versus liquid proliferation media on EtoBox?
Somatic embryogenesis in two Gossypium hirsutum genotypes on semi-solid versus liquid proliferation media by Nick J. Gawel; Carol D. Robacker is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Somatic embryogenesis in two Gossypium hirsutum genotypes on semi-solid versus liquid proliferation media about?
A comparison of semi-solid vs. liquid embryo proliferation media was made using two Gossypium hirsutum L. genotypes (Coker 312 and T25) and two callus initiation media. Sections of petioles from mature, flowering plants were cultured on two modified Murashige and Skoog media. Medium 1 included 4.0 mg 1l NAA and 1.0 mgl ~ kinetin; medium 2 contained 0.1 mg 1-~ 2,4-D and 0. I mg I-l kinetin. After six weeks, callus was removed from each explant and divided in half. One callus portion was placed in liquid proliferation medium and the other on semi-solid (0.2% Gelrite) proliferation medium. Composition of proliferation medium was identical to that of initiation medium, except no growth regulators were added. Embryos were counted after eight weeks. The percentage of explants forming callus was influenced by genotype/initiation medium combination. Analysis of variance procedures revealed significant variability for callus initiation media, proliferation media (semi-solid or liquid), and an initiation medium x genotype interaction. Paired t-tests indicated that more embryos were produced in liquid proliferation medium (227.3 embryos/culture) than on semi-solid proliferation medium (134.6
Who reads Somatic embryogenesis in two Gossypium hirsutum genotypes on semi-solid versus liquid proliferation media?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Nick J. Gawel; Carol D. Robacker
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0167-6857)
- Published
- 1990
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)