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Human herpesvirus type 7 in blood donors: Detection by the polymerase chain reaction by F. Wilborn; C. A. Schmidt; F. Lorenz; R. Peng; H. Gelderblom; D. Huhn; Prof. Dr. Wolfgang Siegert is a Medicine article available to read on EtoBox.

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## Abstract In order to evaluate the prevalence of human herpesvirus type 7 (HHV‐7) in adult blood donors oral lavage fluid, bum coat, and uring samples from 112 persons were examined by the polymerase chain reaction (PCR) at one time point. In addition, 11 donors were studied longitudinally over 11 weeks. When the results of the initial and the longitudinal study were combined HHV‐7 DNA was found in samples from 109 of 112 (97.3%) adult blood donors. On the basis of different sensitivity levels of the first and the nested PCR differences were detected in the viral DNA load in the samples. It was found that lavage fluid regularly carried significantly higher DNA concentrations than buffy coat. Out of 112 donors, 102 (91.1%) and 8 (7.1%) were positive in the first, less sensitive PCR in lavage fluid and buffy coat, respectively (__P__<.0001). After nested PCR, 107 (95.5%) and 74 (66.1%) were positive in lavage fluid and buffy coat, respectively (__P__<.0001). Urine samples were found positive only sporadically. The longitudinal study showed that the oral lavage fluid of most of the donors consistently carried HHV‐7 over up to 53 weeks, whereas buffy coat samples were positive less o

Who reads Human herpesvirus type 7 in blood donors: Detection by the polymerase chain reaction?

Author

F. Wilborn; C. A. Schmidt; F. Lorenz; R. Peng; H. Gelderblom; D. Huhn; Prof. Dr. Wolfgang Siegert

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

1995

Language

EN

Field

Medicine (Health Sciences)