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X-ray Structure Determination at Low Resolution by Axel T. Brunger; Byron DeLaBarre; Jason M. Davies; William I. Weis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is X-ray Structure Determination at Low Resolution about?

As an example of structure determination in the 3.5-4.5 A resolution range, crystal structures of the ATPase p97/VCP, consisting of an N-terminal domain followed by a tandem pair of ATPase domains (D1 and D2), are discussed. The structures were originally solved by molecular replacement with the high-resolution structure of the N-D1 fragment of p97/VCP, whereas the D2 domain was manually built using its homology to the D1 domain as a guide. The structure of the D2 domain alone was subsequently solved at 3 A resolution. The refined model of D2 and the high-resolution structure of the N-D1 fragment were then used as starting models for re-refinement against the low-resolution diffraction data for full-length p97. The re-refined full-length models showed significant improvement in both secondary structure and R values. The free R values dropped by as much as 5% compared with the original structure refinements, indicating that refinement is meaningful at low resolution and that there is information in the diffraction data even at approximately 4 A resolution that objectively assesses the quality of the model. It is concluded that de novo model building is problematic at low resolution

Who reads X-ray Structure Determination at Low Resolution?

Author

Axel T. Brunger; Byron DeLaBarre; Jason M. Davies; William I. Weis

Publisher

International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)