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Molecular cytogenetic analysis of a nontumorigenic human breast epithelial cell line that eventually turns tumorigenic: Validation of an analytical approach combining karyotyping, comparative genomic hybridization, chromosome painting, and single-locus fluorescence in situ hybridization by Kirsten Vang Nielsen; Erik Niebuhr; Bent Ejlertsen; Søren Holstebroe; Mogens Winkel Madsen; Per Briand; Henning T. Mouridsen; Lars Bolund is a Medicine article available to read on EtoBox.

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The immortalized, nontumorigenic human breast epithelial cell line HMT-3522 has been used as a model for premalignant and, eventually, malignant development. During cultivation, the karyotype evolution was followed. At an early stage, a very long constant phase showed a near-diploid karyotype, with only five marker chromosomes. DNA from this phase was used for comparative genomic hybridization (CGH) analysis, confirming a previously known MYC amplification, and the integration sites were subsequently determined by single-locus fluorescence in situ hybridization (FISH). Furthermore, gains of 5q22-qter and 20q11-qter and deletion of most of chromosome 6 (6p23-qter) were detected by CGH. Because of uncertainty about some of the indicated changes, including a deletion of 1p35-pter, the CGH findings were investigated more closely by chromosome painting, leading to a revision of the karyotype: 45,XX,del(1)(p35),26,dup(8)(pter=qter::qter=q24),der(12) t(6;12)(p23; p13),der(14)t(5;14)(q22;q32.3),der(17)t(8;17;20)(17pter=17q25::8qter=8q23::8q24=8qter::8q24=8qter:: 8q23=8q24.1::20q11=20qter). Some karyotypic changes were confirmed by CGH; others had to be revised; and, in the 1p35 region, cla

Who reads Molecular cytogenetic analysis of a nontumorigenic human breast epithelial cell line that eventually turns tumorigenic: Validation of an analytical approach combining karyotyping, comparative genomic hybridization, chromosome painting, and single-locus fluorescence in situ hybridization?

Author

Kirsten Vang Nielsen; Erik Niebuhr; Bent Ejlertsen; Søren Holstebroe; Mogens Winkel Madsen; Per Briand; Henning T. Mouridsen; Lars Bolund

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 1045-2257)

Published

1997

Language

EN

Field

Medicine (Life Sciences)