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Can I read Carrier-free Zone Electrophoresis, Displacement Electrophoresis and Isoelectric Focusing in a High-performance Electrophoresis Apparatus on EtoBox?
Carrier-free Zone Electrophoresis, Displacement Electrophoresis and Isoelectric Focusing in a High-performance Electrophoresis Apparatus by Stellan Hjertén; Karin Elenbring; Ferenc Kilár; Jia-Li Liao; Albert J.C. Chen; Christopher J. Siebert; Ming-De Zhu is a Chemistry article available to read on EtoBox.
What is Carrier-free Zone Electrophoresis, Displacement Electrophoresis and Isoelectric Focusing in a High-performance Electrophoresis Apparatus about?
A characteristic feature of high-performance electrophoresis (HPE), the electrophoretic counterpart of high-performance liquid chromatography (HPLC), is that the separation chamber is a thin-walled, narrow-bore (0.05-0.3 mm) glass or fusedsilica capillary tube for rapid dissipation of the Joule heat in order to minimize thermal zone deformation even at high field strengths. This paper is centered around the usefulness of HPE for separation in a carrier-free medium (i.e., in buffer alone) and deals with both zone electrophoresis, isoelectric focusing and displacement electrophoresis. Examples are given of analytical and micropreparative separations of inorganic and organic ions, proteins, viruses and bacteria. The run times are 5-30 min. Discontinuous buffer systems have up to now been used exclusively for the separation of proteins by electrophoresis in polyacrylamide gels ("disc electrophoresis"). However, the Ornstein and Davis discontinuous buffer system has been modified to adapt it to carrier-free zone electrophoresis in order to achieve automatic sharpening of the starting zone. Very high resolution of serum proteins was obtained when they were subjected to free high-performa
Who reads Carrier-free Zone Electrophoresis, Displacement Electrophoresis and Isoelectric Focusing in a High-performance Electrophoresis Apparatus?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Stellan Hjertén; Karin Elenbring; Ferenc Kilár; Jia-Li Liao; Albert J.C. Chen; Christopher J. Siebert; Ming-De Zhu
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1987
- Language
- EN
- Field
- Chemistry (Physical Sciences)