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Effect of IL‐1β, PGE~2~, and TGF‐β1 on the expression of OPG and RANKL in normal and osteoporotic primary human osteoblasts by Susana Jurado; Natalia Garcia‐Giralt; Adolfo Díez‐Pérez; Pedro Esbrit; Guy Yoskovitz; Lídia Agueda; Roser Urreizti; Lluís Pérez‐Edo; Guillem Saló; Leonardo Mellibovsky; Susana Balcells; Daniel Grinberg; Xavier Nogués is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The __RANKL__/__RANK__/__OPG__ pathway is essential for bone remodeling regulation. Many hormones and cytokines are involved in regulating gene expression in most of the pathway components. Moreover, any deregulation of this pathway can alter bone metabolism, resulting in loss or gain of bone mass. Whether osteoblasts from osteoporotic and nonosteoporotic patients respond differently to cytokines is unknown. The aim of this study was to compare the effect of interleukin (IL)‐1β, proftaglandin E~2~ (PGE~2~), and transforming growth factor‐β1 (TGF‐β1) treatments on __OPG__ and __RANKL__ gene expression in normal (n = 11) and osteoporotic (n = 8) primary osteoblasts. __OPG__ and __RANKL__ mRNA levels of primary human osteoblastic (hOB) cell cultures were assessed by real‐time PCR. In all cultures, __OPG__ mRNA increased significantly in response to IL‐1β treatment and decreased in response to TGF‐β1 whereas PGE~2~ treatment had no effect. __RANKL__ mRNA levels were significantly increased by all treatments. Differences in __OPG__ and __RANKL__ responses were observed between osteoporotic and nonosteoporotic hOB: in osteoporotic hOB, the __OPG__ response to IL‐1β treatment

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Author

Susana Jurado; Natalia Garcia‐Giralt; Adolfo Díez‐Pérez; Pedro Esbrit; Guy Yoskovitz; Lídia Agueda; Roser Urreizti; Lluís Pérez‐Edo; Guillem Saló; Leonardo Mellibovsky; Susana Balcells; Daniel Grinberg; Xavier Nogués

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)