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Subsurface cisterns in α‐motoneurons of the rat and cat: Immunohistochemical detection with antibodies against connexin32 by T. Yamamoto; E. L. Hertzberg; Dr. J. I. Nagy is a Neuroscience article available to read on EtoBox.

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A monoclonal antibody against amino acids 224-234 of the gap junction protein connexin32 was found by immunohistochemistry to label subsurface cisterns (SSCs) in alpha-motoneurons of the rat (Yamamoto et al., 1990) and was used here to document by light (LM) and electron microscopy (EM) the appearance of immunoreactive SSCs in motoneurons of the rat and cat. This antibody and a polyclonal antibody against connexin32 labelled gap junctions in rat liver as well as SSCs in facial motoneurons. By LM, SSCs were seen as labelled puncta on motoneuronal perikarya and proximal dendrites. In the rat, they appeared to be present on all motoneurons at cranial and spinal levels, but varied considerably in size and number among motor nuclei. Labelled SSCs were the smallest and most sparse in motoneurons of the dorsal vagal motor nucleus, moderate in size and most numerous in the trochlear, oculomotor, and trigeminal motor nuclei, and largest though less densely distributed in spinal motoneurons. Dendrites were seen to contain SSCs for distances of up to 230 pm from their soma1 origm. Labelling within individual SSCs seen en face consisted of either numerous small puncta or linear arrays of immun

Who reads Subsurface cisterns in α‐motoneurons of the rat and cat: Immunohistochemical detection with antibodies against connexin32?

Author

T. Yamamoto; E. L. Hertzberg; Dr. J. I. Nagy

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0887-4476)

Published

1991

Language

EN

Field

Neuroscience (Life Sciences)