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New application of air-drying techniques for studying Ephemeroptera and Plecoptera eggs by scanning electron microscopy by Nicolás Ubero-Pascal; José Manuel Fortuño; María de Los Ángeles Puig is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Hexamethyldisilizane (HMDS) and tetramethylsilane are organic compounds that are volatile at ambient temperature and which can therefore be used for air-drying biological samples for SEM studies. The techniques using these compounds provide results that are comparable with those obtained by critical point drying, but which involve a very simple process that saves time and money. Both techniques were applied to SEM studies of Ephemeroptera and Plecoptera eggs in order to assess their suitability as alternative methods to critical point drying for these kinds of biological material. The results show no morphological differences between eggs HMDS air-dried and critical point-dried.

Who reads New application of air-drying techniques for studying Ephemeroptera and Plecoptera eggs by scanning electron microscopy?

Author

Nicolás Ubero-Pascal; José Manuel Fortuño; María de Los Ángeles Puig

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1059-910X)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)