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Molecular characterization of a trafficking organelle: Dissecting the axonal paths of calsyntenin-1 transport vesicles by Martin Steuble; Bertran Gerrits; Alexander Ludwig; José María Mateos; Tu-My Diep; Mitsuo Tagaya; Alexander Stephan; Philipp Schätzle; Beat Kunz; Peter Streit; Peter Sonderegger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Kinesin motors play crucial roles in the delivery of membranous cargo to its destination and thus for the establishment and maintenance of cellular polarization. Recently, calsyntenin‐1 was identified as a cargo‐docking protein for Kinesin‐1‐mediated axonal transport of tubulovesicular organelles along axons of central nervous system neurons. To further define the function of calsyntenin‐1, we immunoisolated calsyntenin‐1 organelles from murine brain homogenates and determined their proteome by MS. We found that calsyntenin‐1 organelles are endowed with components of the endosomal trafficking machinery and contained the β‐amyloid precursor protein (APP). Detailed biochemical analyses of calsyntenin‐1 immunoisolates in conjunction with immunocytochemical colocalization studies with cultured hippocampal neurons, using endosomal marker proteins for distinct subcompartments of the endosomal pathways, indicated that neuronal axons contain at least two distinct, nonoverlapping calsyntenin‐1‐containing transport packages: one characterized as early‐endosomal, APP positive, the other as recycling‐endosomal, APP negative. We postulate that calsyntenin‐1 acts as a general mediato
Who reads Molecular characterization of a trafficking organelle: Dissecting the axonal paths of calsyntenin-1 transport vesicles?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Martin Steuble; Bertran Gerrits; Alexander Ludwig; José María Mateos; Tu-My Diep; Mitsuo Tagaya; Alexander Stephan; Philipp Schätzle; Beat Kunz; Peter Streit; Peter Sonderegger
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)