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Structural and functional characterization of rhizosphere actinomycetes of major crop plants under shifting cultivation practice in Northeast India by Marcy D. Momin; Ngangbam Somen Singh; Awadhesh Kumar; Shri Kant Tripathi is a Agricultural and Biological Sciences article available to read on EtoBox.

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This paper aims to study the rhizosphere soils of major traditional crop plants under shifting cultivation of Mizoram for diversity and morphological characterization of actinomycetes, and screening of their salt-tolerance and PGPR activities. The rhizosphere soils from six major crops with adequate replications were collected from two locations (i.e. Reiek and Lengpui, about 30 km apart) of Mamit District of Mizoram. Rhizospheric actinomycetes were isolated by serial dilution method. Purified actinomycetes were screened for their salt resistance under different dilutions (i.e. 0, 50, 70,100, 150, 180, 200, 250, 300 g/l) of NaCl concentrations and their PGPR activities for the production of IAA, siderophores and ammonia. Genomic DNA extraction and their 16SrRNA sequencing of most potential strain AB726 were characterized. Further, rhizospheric soils were studied for their soil physico-chemical properties. A total of 26 rhizospheric actinomycetes were purified and identified. Out of which, five isolates (strain AB726, SKT127, SKT337, SKT97, SKT407) were positive to salt tolerance and one (strain AB726) was strongly positive to all the tested PGPR properties. However, strains SKT725,

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Author

Marcy D. Momin; Ngangbam Somen Singh; Awadhesh Kumar; Shri Kant Tripathi

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)