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Continuous Enzymatic Production of Xylitol with Simultaneous Coenzyme Regeneration in a Charged Membrane Reactor by Bernd Nidetzky; Wilfried Neuhauser; Dietmar Haltrich; Klaus D. Kulbe is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Continuous Enzymatic Production of Xylitol with Simultaneous Coenzyme Regeneration in a Charged Membrane Reactor about?

We have developed a new process for the production of xylitol from D-xylose by enzyme technology. An NADHdependent xylose reductase (XR) from Candida tenuis catalyzes the reduction of xylose, which is coupled to enzymatic oxidations of o-glucose or D-xylose by glucose dehydrogenase from Bacillus cereus to make achievable an up to 10,000-fold regeneration of NADH per cycle of discontinuous conversion. Using a simple kinetic model as a tool for process optimization, suitable conditions with regard to performance and stability of the rnulticomponent reaction system were established, and 300 g/L of substrate could be converted in yields above 96% in one single batch reaction. Due to selective and over 98% complete retention of the native coenzyme by negatively charged nanofiltration membranes used in a continuously operated enzyme reactor, a specific productivity of 80 g xylitol per liter, day, and kilounit of XR was maintained over the 150-h reaction time with only a single dosage of NADH. 0 1996 John Wiley & Sons, Inc.

Who reads Continuous Enzymatic Production of Xylitol with Simultaneous Coenzyme Regeneration in a Charged Membrane Reactor?

Author

Bernd Nidetzky; Wilfried Neuhauser; Dietmar Haltrich; Klaus D. Kulbe

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)