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Evaluation of Cytotoxic Effect of Photodynamic Therapy in Combination with Electroporation in Vitro by J. Labanauskiene; J. Gehl; J. Didziapetriene is a Engineering article available to read on EtoBox.

What is Evaluation of Cytotoxic Effect of Photodynamic Therapy in Combination with Electroporation in Vitro about?

Under the influence of electric pulses cells undergo membrane electroporation (EP), which results in increased permeability of the membrane to exogenous compounds. EP is applied in oncology as a method to enhance delivery of anticancer drugs. For that reason it was essential to combine photodynamic tumor therapy (PDT)-the cancer treatment method based on the use of photosensitizers that localize selectively in malignant tumors and become cytotoxic when exposed to light, and EP, with the aim to enhance the delivery of photosensitizers into the tumor and therefore to increase the efficacy of PDT. Thus, the aim of study was to evaluate the cytotoxic effect of PDT in combination with EP. A Chinese hamster lung fibroblast cell line (DC-3F) was used. The cells were affected by photosensitizers chlorin e 6 (C e 6 ) at the dose of 10 μg/ml and aluminium phthalocyanine tetrasulfonate (AlPcS4) at the dose of 50 μg/ml. Immediately after adding of photosensitizers the cells were electroporated with 8 electric pulses at 1200 V/cm intensity, 0.1 ms duration, 1 Hz frequency. Then, after 20 min of incubation the cells were irradiated using a light source-a visible light passing through a filter (K

Who reads Evaluation of Cytotoxic Effect of Photodynamic Therapy in Combination with Electroporation in Vitro?

Author

J. Labanauskiene; J. Gehl; J. Didziapetriene

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1567-5394)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)