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Can I read Studies of Membrane Formation in Tetrahymena pyriformis on EtoBox?
Studies of Membrane Formation in Tetrahymena pyriformis by Papasani V. Subbaiah; Guy A. Thompson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Studies of Membrane Formation in Tetrahymena pyriformis about?
An experimental system has been developed to analyze the synthesis and intracellular deployment of proteins in growing cells of Tefrahymena pyriformis. There are marked differences in the rates at which radioactive proteins appear in various parts of the cell, including certain membranes, following [3H]leucine pulse-chase labeling experiments. Proteins labeled during a 5-min exposure to the isotope require approximately 2 hours for intracellular dissemination. Polyacrylamide gel electrophoresis indicated that all individual proteins of a particular cell fraction accumulate radioactivity at equivalent rates, except in the case of cilia. Further subfractionation of purified cilia yielded three components: matrix, axonemes, and membranes. The latter fraction was found to have a relatively simple protein composition involving only four major constituents. The matrix fraction, which had the highest specific radioactivity immediately following the aH pulse, appeared to contain new proteins being conveyed to membranes and axonemes. By 2 hours following the chase, all three subfractions of the cilia had nearly equivalent specific radioactivities. Radioactive membrane lipids also seem to be
Who reads Studies of Membrane Formation in Tetrahymena pyriformis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Papasani V. Subbaiah; Guy A. Thompson
- Publisher
- Elsevier BV
- Published
- 1974
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)