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High-efficiency gene editing in Anopheles sinensis using ReMOT control by Xiao-lin Yang; Xia Ling; Quan Sun; Pin-pin Qiu; Kai Xiang; Jun-feng Hong; Shu-lin He; Jie Chen; Xin Ding; Hai Hu; Zheng-bo He; Cao Zhou; Bin Chen; Liang Qiao is a scholarly article available to read on EtoBox.

What is High-efficiency gene editing in Anopheles sinensis using ReMOT control about?

## Abstract CRISPR/Cas9-mediated gene editing provides an effective method for deciphering the molecular mechanisms underlying mosquito development and mosquito-borne disease transmission, as well as for exploring genetic control strategies. However, delivering the Cas9 ribonucleoprotein complex by embryo injection to produce genetic modifications is challenging, is mostly confined to model mosquitoes and specialized laboratories, and has low editing efficiency. Here, we established an effective Receptor-Mediated Ovary Transduction of Cargo (ReMOT) control method, enabling the introduction of heritable mutations intoAnopheles sinensis, the major malaria vector in China and Southeast Asia, via the injection of female adult mosquitoes. Injection of a mixture of P2C-DsRed and saponin resulted in red fluorescence in the ovaries, with a 100% success rate. Using this system, we knocked-out the pigment synthesis genes,AswhiteandAsyellow, using injected wild-type (WT) females mated with WT males, resulting in the highest efficiency of gene editing among mosquitoes under the same mating conditions. Furthermore, the gene-editing efficiency was increased by at least 2.1-fold using injected WT

Author

Xiao-lin Yang; Xia Ling; Quan Sun; Pin-pin Qiu; Kai Xiang; Jun-feng Hong; Shu-lin He; Jie Chen; Xin Ding; Hai Hu; Zheng-bo He; Cao Zhou; Bin Chen; Liang Qiao

Publisher

Cold Spring Harbor Laboratory

Published

2023