Can I read RNA-responsive elements for eukaryotic translational control on EtoBox?

RNA-responsive elements for eukaryotic translational control by Evan M. Zhao; Angelo S. Mao; Helena de Puig; Kehan Zhang; Nathaniel D. Tippens; Xiao Tan; F. Ann Ran; Isaac Han; Peter Q. Nguyen; Emma J. Chory; Tiffany Y. Hua; Pradeep Ramesh; David B. Thompson; Crystal Yuri Oh; Eric S. Zigon; Max A. English; James J. Collins is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is RNA-responsive elements for eukaryotic translational control about?

The ability to control translation of endogenous or exogenous RNAs in eukaryotic cells would facilitate a variety of biotechnological applications. Current strategies are limited by low fold changes in transgene output and the size of trigger RNAs (trRNAs). Here we introduce eukaryotic toehold switches (eToeholds) as modular riboregulators. eToeholds contain internal ribosome entry site sequences and form inhibitory loops in the absence of a specific trRNA. When the trRNA is present, eToeholds anneal to it, disrupting the inhibitory loops and allowing translation. Through optimization of RNA annealing, we achieved up to 16-fold induction of transgene expression in mammalian cells. We demonstrate that eToeholds can discriminate among viral infection status, presence or absence of gene expression and cell types based on the presence of exogenous or endogenous RNA transcripts.

Who reads RNA-responsive elements for eukaryotic translational control?

Author

Evan M. Zhao; Angelo S. Mao; Helena de Puig; Kehan Zhang; Nathaniel D. Tippens; Xiao Tan; F. Ann Ran; Isaac Han; Peter Q. Nguyen; Emma J. Chory; Tiffany Y. Hua; Pradeep Ramesh; David B. Thompson; Crystal Yuri Oh; Eric S. Zigon; Max A. English; James J. Collins

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)