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Identification and Quantification of Actin Isoforms in Vertebrate Cells and Tissues by C. A. Otey; M. H. Kalnoski; J. C. Bulinski is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Identification and Quantification of Actin Isoforms in Vertebrate Cells and Tissues about?

The cytoskeletal protein actin exists in vertebrates as six different isoforms, which are difficult to identify conclusively because of a high degree (> 90%) of overall sequence homology. We have used IEF immunoblotting in combination with a panel of isoform-specific and -selective antibodies to analyze the actin isoform composition of nine tissues from adult rat. In three nonmuscle tissues (lung, spleen, and testis), we detected a previously unreported isoform that we identified as smooth muscle a. The IEF immunoblot technique was also used to quantify the proportions of the isoforms expressed in these nine rat tissues.

Who reads Identification and Quantification of Actin Isoforms in Vertebrate Cells and Tissues?

Author

C. A. Otey; M. H. Kalnoski; J. C. Bulinski

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1987

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)