Can I read Electrophoresis in Gels Containing Zwitterionic Groups on EtoBox?

Electrophoresis in Gels Containing Zwitterionic Groups by Leonard M. Hjelmeland; Stig Allenmark; Birgit An der Lan; B. Andrew Jackiw; Nga Y. Nguyen; Andreaas Chrambach is a Engineering article available to read on EtoBox.

What is Electrophoresis in Gels Containing Zwitterionic Groups about?

## Abstract A zwitterionic methacrylamide derivative, (3‐sulfopropyl)dimethy1(3‐methacrylamidopropyl) ammonium inner salt (MAPS), was synthesized and then copolymerized with N,N′‐methylenebisacrylamide (Bis) to make gels carrying covalently bound sulfobetaine groups. Gels were also prepared with the addition of (3‐sulfopropyl)trimethyl ammonium inner salt (TMAPS) to Bis‐crosslinked polyacrylamide prior to polymerization. The properties of these gels in gel electrophoresis and electrofocusing were tested and compared with those of Bis‐crosslinked polyacrylamide. In MAPS gels the absolute migration velocities of proteins and dyes at either polarity of migration were decreased. Anionic species were much more strongly retarded than cationic species. Ferguson plots (based on protein mobilities relative to dye in a continuous buffer) of catalase at pH 4 obtained on MAPS gels had a similar slope but lower y‐intercept compared with those on equivalent polyacrylamide gels, indicating that the available (effective) net charge on the protein was decreased because it interacts with the zwitterionic charge on the gel. Similarly, the addition of TMAPS to polyacrylamide decreased the relative cat

Who reads Electrophoresis in Gels Containing Zwitterionic Groups?

Author

Leonard M. Hjelmeland; Stig Allenmark; Birgit An der Lan; B. Andrew Jackiw; Nga Y. Nguyen; Andreaas Chrambach

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)

Published

1981

Language

EN

Field

Engineering (Physical Sciences)