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Targeting Bacterial Membranes: Identification of Pseudomonas aeruginosaD-Arabinose-5P Isomerase and NMR Characterisation of its Substrate Recognition and Binding Properties by Dr. Cristina Airoldi; Dr. Silvia Sommaruga; Silvia Merlo; Dr. Paola Sperandeo; Prof. Laura Cipolla; Prof. Alessandra Polissi; Prof. Francesco Nicotra is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Targeting Bacterial Membranes: Identification of Pseudomonas aeruginosaD-Arabinose-5P Isomerase and NMR Characterisation of its Substrate Recognition and Binding Properties about?
## Abstract The identification and characterisation of __Pseudomonas aeruginosa__ KdsD (Pa‐KdsD), a D‐arabinose‐5P isomerase involved in the biosynthesis of 3‐deoxy‐D‐__manno__‐oct‐2‐ulosonic acid and thus of lipopolysaccharide (LPS), are reported. We have demonstrated that KdsD is essential for __P. aeruginosa__ survival and thus represents a key target for the development of novel antibacterial drugs. The key amino acid residues for protein activity have been identified. The structural requirements for substrate recognition and binding have been characterised for the wild‐type protein, and the effect of mutations of the key residues on catalytic activity and binding have been evaluated by saturation transfer difference (STD) NMR spectroscopy. Our data provide important structural information for the rational design of new KdsD inhibitors as potential antibacterial drugs.
Who reads Targeting Bacterial Membranes: Identification of Pseudomonas aeruginosaD-Arabinose-5P Isomerase and NMR Characterisation of its Substrate Recognition and Binding Properties?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Dr. Cristina Airoldi; Dr. Silvia Sommaruga; Silvia Merlo; Dr. Paola Sperandeo; Prof. Laura Cipolla; Prof. Alessandra Polissi; Prof. Francesco Nicotra
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1439-4227)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)