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Can I read Proteomic profiling of soft tissue sarcomas with SWATH mass spectrometry on EtoBox?
Proteomic profiling of soft tissue sarcomas with SWATH mass spectrometry by Martina Milighetti; Lukas Krasny; Alex T.J. Lee; Gabriele Morani; Cornelia Szecsei; Yingtong Chen; Nafia Guljar; Frank McCarthy; Christopher P. Wilding; Amani Arthur; Cyril Fisher; Ian Judson; Khin Thway; Maggie C.U. Cheang; Robin L. Jones; Paul H. Huang is a Chemistry article available to read on EtoBox.
What is Proteomic profiling of soft tissue sarcomas with SWATH mass spectrometry about?
Soft tissue sarcomas (STS) are a group of rare and heterogeneous cancers. While large-scale genomic and epigenomic profiling of STS have been undertaken, proteomic analysis has thus far been limited. Here we utilise sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS) for proteomic profiling of formalin fixed paraffin embedded (FFPE) specimens from a cohort of STS patients (__n__ = 36) across four histological subtypes (leiomyosarcoma, synovial sarcoma, undifferentiated pleomorphic sarcoma and dedifferentiated liposarcoma). We quantified 2951 proteins across all cases and show that there is a significant enrichment of gene sets associated with smooth muscle contraction in leiomyosarcoma, RNA splicing regulation in synovial sarcoma and leukocyte activation in undifferentiated pleomorphic sarcoma. We further identified a subgroup of STS cases that have a distinct expression profile in a panel of proteins, with worse survival outcomes when compared to the rest of the cohort. Our study highlights the value of comprehensive proteomic characterisation as a means to identify histotype-specific STS profiles that describe key biological pathways
Who reads Proteomic profiling of soft tissue sarcomas with SWATH mass spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Martina Milighetti; Lukas Krasny; Alex T.J. Lee; Gabriele Morani; Cornelia Szecsei; Yingtong Chen; Nafia Guljar; Frank McCarthy; Christopher P. Wilding; Amani Arthur; Cyril Fisher; Ian Judson; Khin Thway; Maggie C.U. Cheang; Robin L. Jones; Paul H. Huang
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Chemistry (Physical Sciences)