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Can I read Space shuttle flight (STS-45) of L8 myoblast cells results in the isolation of a nonfusing cell line variant on EtoBox?
Space shuttle flight (STS-45) of L8 myoblast cells results in the isolation of a nonfusing cell line variant by David A. Kulesh; Loraine H. Anderson; Bernard Wilson; Ericka J. Otis; William J. Mehm; Diane M. Elgin; Michael J. Barker; George P. Kearney is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Space shuttle flight (STS-45) of L8 myoblast cells results in the isolation of a nonfusing cell line variant about?
Myoblast cell cultures have been widely employed in conventional (Ig) studies of biological processes because characteristics of intact muscle can be readily observed in these cultured cells. We decided to investigate the effects of spaceflight on muscle by utilizing a well characterized myoblast cell line (L8 rat myoblasts) as cultured in the recently designed Space Tissue Loss Flight Module "A" (STL-A). The STL-A is a "state of the art," compact, fully contained, automated cell culture apparatus which replaces a single mid-deck locker on the Space Shuttle. The L8 cells were successfully flown in the STL-A on the Space Shuttle STS-45 mission. Upon return to earth, reculturing of these spaceflown L8 cells (L8SF) resulted in their unexpected failure to fuse and differentiate into myotubes. This inability of the L8SF cells to fuse was found to be a permanent phenotypic alteration. Scanning electron microscopic examination of L8SF cells growing at Ig on fibronectin-coated polypropylene fibers exhibited a strikingly different morphology as compared to control cells. In addition to their failure to fuse into myotubes, L8SF cells also piled up on top of each other. When assayed in fusion
Who reads Space shuttle flight (STS-45) of L8 myoblast cells results in the isolation of a nonfusing cell line variant?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- David A. Kulesh; Loraine H. Anderson; Bernard Wilson; Ericka J. Otis; William J. Mehm; Diane M. Elgin; Michael J. Barker; George P. Kearney
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 1994
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)