Can I read Stress-wave-assisted Transport Through the Plasma Membrane in Vitro on EtoBox?

Stress-wave-assisted Transport Through the Plasma Membrane in Vitro by McAuliffe, Daniel J.; Lee, Shun; Flotte, Thomas J.; Doukas, Apostolos G. is a Medicine article available to read on EtoBox.

What is Stress-wave-assisted Transport Through the Plasma Membrane in Vitro about?

## Background and Objective : Laser-induced stress waves have been shown to alter the permeability of the plasma membrane without affecting cell viability. The aim of the work reported here was to quantify the molecular uptake by cell cultures in vitro and determine optimal stress-wave parameters. Study Design/Materials and Methods: Human peripheral blood mononuclear cells were exposed to laser-induced stress waves in an experimental arrangement that eliminated interference from ancillary effects such as plasma, heat, or cavitation. A radiolabeled compound (tritiated thymidine) was used as the probe. Results: Stress waves enhanced the diffusion of tritiated thymidine by inducing a transient permeabilization of the plasma membrane. Furthermore, maximum intracellular concentration (2×10 5 thymidine molecules/cell or 10% of the extracellular concentration) was reached with only 2-3 stress waves. Conclusion: Laser-induced stress waves provide an efficient method for delivering molecules through the plasma membrane into the cytoplasm of cells.

Who reads Stress-wave-assisted Transport Through the Plasma Membrane in Vitro?

Author

McAuliffe, Daniel J.; Lee, Shun; Flotte, Thomas J.; Doukas, Apostolos G.

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0196-8092)

Published

1997

Language

EN

Field

Medicine (Health Sciences)