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Can I read EMP3 as a prognostic biomarker correlates with EMT in GBM on EtoBox?
EMP3 as a prognostic biomarker correlates with EMT in GBM by Li Li; Siyu Xia; Zitong Zhao; Lili Deng; Hanbing Wang; Dongbo Yang; Yizhou Hu; Jingjing Ji; Dayong Huang; Tao Xin is a Medicine article available to read on EtoBox.
What is EMP3 as a prognostic biomarker correlates with EMT in GBM about?
## Background Glioblastoma (GBM) is the most aggressive malignant central nervous system tumor with a poor prognosis.The malignant transformation of glioma cells via epithelial-mesenchymal transition (EMT) has been observed as a main obstacle for glioblastoma treatment. Epithelial membrane protein 3 (__EMP3__) is significantly associated with the malignancy of GBM and the prognosis of patients. Therefore, exploring the possible mechanisms by which __EMP3__ promotes the growth of GBM has important implications for the treatment of GBM. ## Methods We performed enrichment and correlation analysis in 5 single-cell RNA sequencing datasets. Differential expression of __EMP3__ in gliomas, Kaplan–Meier survival curves, diagnostic accuracy and prognostic prediction were analyzed by bioinformatics in the China Glioma Genome Atlas (CGGA) database and The Cancer Genome Atlas (TCGA) database. __EMP3__-silenced U87 and U251 cell lines were obtained by transient transfection with siRNA. The effect of __EMP3__ on glioblastoma proliferation was examined using the CCK-8 assay. Transwell migration assay and wound healing assay were used to assess the effect of EMP3 on glioblastoma migration. Finally,
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- Author
- Li Li; Siyu Xia; Zitong Zhao; Lili Deng; Hanbing Wang; Dongbo Yang; Yizhou Hu; Jingjing Ji; Dayong Huang; Tao Xin
- Publisher
- BioMed Central
- Published
- 2024
- Language
- EN
- Field
- Medicine (Health Sciences)