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FastTomo: A SerialEM Script for Collecting Electron Tomography Data by Albert Xu; Chen Xu is a scholarly article available to read on EtoBox.

What is FastTomo: A SerialEM Script for Collecting Electron Tomography Data about?

## Abstract FastTomo is a SerialEM script for collecting tilted specimen images in transmission electron microscopes to be further used in tomographic reconstruction. It achieves a speedup over conventional tracking methods by minimizing the usage of off-target tracking shots, and instead applies proportional control to the specimen images. Movement in the Z coordinate is estimated prior to each tilt series in a separate calibration routine. Overall, this method is fast and reliable when the field of view is at least 1 um, and can tolerate minor errors in setting eucentric height. The implemented tilt series schemes include the unidirectional, bidirectional, and dose-symmetric schemes.

Author

Albert Xu; Chen Xu

Publisher

Cold Spring Harbor Laboratory

Published

2021

Language

EN