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Can I read α2-Macroglobulin Traps a Proteinase in the Midregion of Its Arms on EtoBox?
α2-Macroglobulin Traps a Proteinase in the Midregion of Its Arms by H Arakawa; M Nishigai; A Ikai is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is α2-Macroglobulin Traps a Proteinase in the Midregion of Its Arms about?
az-Macroglobulin, one of the major plasma proteinase inhibitors with M. = 720,000, is known to inhibit proteinases of all four classes through the "trap mechanism" (Barrett, A. J., and Starkey, P. M. (1973) Biochem. J. 133,709-724), but the proteinase binding site of aa-macroglobulin has not been identified precisely. We localized bound proteinase molecules on the electron microscopic images of az-macroglobulin, using anti-proteinase IgG. Serratia1 M, = 56,000 proteinase produced by Serratia marcescens was chosen as the antigenic probe in this study because its affinity to specific antibodies was retained in its bound state to az-macroglobulin. Dimers of az-macroglobulin/Mr = 56,000 proteinase complexes cross-linked with anti-M, = 56,000 proteinase IgG were prepared and subjected to electron microscopic observations. The electron microscopic image of az-macroglobulin complexed with M, = 56,000 proteinase had four straight arms with an overall shape looking like the character "H." From the way anti", = 56,000 proteinase IgG linked two cyz-macroglobulins, it was concluded that the proteinase existed in the midregion of one of the arms. This result helps us to form a more concrete vie
Who reads α2-Macroglobulin Traps a Proteinase in the Midregion of Its Arms?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- H Arakawa; M Nishigai; A Ikai
- Publisher
- Elsevier BV
- Published
- 1989
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)