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Improved gas chromatography with electron-capture detection using a reaction pre-column for the determination of blood cyanide: A higher content in the left ventricle of fire victims by Chikatoshi Maseda; Kazuo Matsubara; Hiroshi Shiono is a Chemistry article available to read on EtoBox.

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We developed a head-space method for the determmation of blood cyanide by gas chromatography with electron-capture detection. In this technique, a reaction pre-column, packed with chloramine-T, was used for the conversion of hydrogen cyanide into cyanogen chloride. Since the reaction pre-column eliminated the necessity for trapping hydrogen cyanide from the biological samples, blood cyanide was quickly analysed by acidification only. The reaction pre-column was durable for at least several months. The calibration curve gave good linearity when dichloromethane was used as the internal standard, and the lower detection limit taken from this plot was ca. 0.05 &ml. The relative standard deviation of spiked blood samples was in the range 0 6-3.9%. We determined blood cyanide levels at autopsy m victims who had died from fire using this method. A sigmficantly higher cyanide content was detected in the left ventricular blood than in the right. There was a positive correlation between blood cyanide and carboxylhaemoglobin contents. This simple and sensitive technique could be very useful for the determination of cyanide in various samples.

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Author

Chikatoshi Maseda; Kazuo Matsubara; Hiroshi Shiono

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1989

Language

EN

Field

Chemistry (Physical Sciences)