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Can I read Molecular cloning, characterization and expression profiles of multiple leptin genes and a leptin receptor gene in orange-spotted grouper (Epinephelus coioides) on EtoBox?
Molecular cloning, characterization and expression profiles of multiple leptin genes and a leptin receptor gene in orange-spotted grouper (Epinephelus coioides) by Huixian Zhang; Huapu Chen; Yong Zhang; Shuisheng Li; Danqi Lu; Haifa Zhang; Zining Meng; Xiaochun Liu; Haoran Lin is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Molecular cloning, characterization and expression profiles of multiple leptin genes and a leptin receptor gene in orange-spotted grouper (Epinephelus coioides) about?
Leptin plays key roles in body weight regulation, energy metabolism, food intake, reproduction and immunity in mammals. However, its function in teleosts is still unclear. In the present study, two leptin genes (gLepA and gLepB) and one leptin receptor gene (gLepR) were cloned and characterized in orange-spotted grouper (Epinephelus coioides). The cDNAs of gLepA and gLepB were 671 bp and 684 bp in length, encoding for proteins of 161 amino acid (aa) and 158 aa, respectively. The three-dimensional (3D) structures modeling of gLepA and gLepB showed strong conservation of tertiary structure with that of other vertebrates. The total length of gLepR cDNA was 4242 bp, encoding a protein of 1169 aa which contained all functionally important domains conserved among vertebrate LEPR. Tissue distribution analysis showed that gLepA was highly expressed in cerebellum, liver and ovary, while gLepB mRNA abundantly in the brain regions, as well as in the ovary with some extend. The gLepR was mainly expressed in kidney, head kidney and most of brain regions. Analysis of expression profiles of gLep and gLepR genes during the embryonic stages showed that high expression of gLepR was observed in the b
Who reads Molecular cloning, characterization and expression profiles of multiple leptin genes and a leptin receptor gene in orange-spotted grouper (Epinephelus coioides)?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Huixian Zhang; Huapu Chen; Yong Zhang; Shuisheng Li; Danqi Lu; Haifa Zhang; Zining Meng; Xiaochun Liu; Haoran Lin
- Publisher
- Elsevier BV
- Published
- 2013
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)