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ARS-Aktivität von Kinetoplasten-DNA in Hefezellen by H.-P. R. Gütter; D. A. Maslow; A. A. Kolesnikow; Th. Börner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is ARS-Aktivität von Kinetoplasten-DNA in Hefezellen about?

Different fragments of the maxicircle of the kinetoplast DNA (kpDNA) from C'rithidia oncopelti were cloned (Fig. 1, 2) and tested for ARS-activity (ARS, autonomously replicating sequences). ARS-avitivity was expressed as number of transformed yeast cells per pg plasmid DNA, as number of transformed cells per number of plasmid molecules (tmnsformation efficiency, TE) and as number of transformed cells per kilobasepair of cloned kpDNA (specific transformation efficiency, TES) (Fig. 3, Table 1). All DNA fragments studied ehowed ARS-activity. Large fragments exhibited higher ARS-activities than their smaller subfragments. The number of fragments showing ARS activity and their distribution within the maxicircles (Fig. 4) suggest that there was no strong correlation between sites with ARS-activity and the replication origin of kpDNA.

Who reads ARS-Aktivität von Kinetoplasten-DNA in Hefezellen?

Author

H.-P. R. Gütter; D. A. Maslow; A. A. Kolesnikow; Th. Börner

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Akademie Verlag; Wiley (ISSN 0233-111X)

Published

1985

Language

DE

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)