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Intracellular Cytokine Expression in Whole Blood Preparations from Normals and Patients with Atopic Dermatitis by B. FERRY; P. ANTROBUS; I. HUZICKA; A. FARRELL; A. LANE; H. CHAPEL is a Immunology and Microbiology article available to read on EtoBox.

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## SUMMARY In recent years, the importance of characterizing the role of cytokines in a wide range of clinical conditions has resulted in development of new methods to assess cytokine expression in clinical samples. The use of anti-cytokine MoAbs and flow cytometry to detect cytokines intracellularly at the single-cell level has the potential to quantify cytokine production in different diseases. For this technique to be useful in a clinical setting, rapid throughput of clinical samples and a cheap, reliable assay would be required, therefore the development of the above technique using unseparated whole blood samples would be advantageous. Using this technique, only one study to date (Maino et al., 1996) has used unseparated whole blood as the source of cells for detecting intracellular cytokines. In clinical practice, whole blood may be optimal, since this most closely approximates conditions in vivo: as no purification of blood mononuclear cells is required, very little blood is needed to detect a number of cytokines simultaneously in various lymphocyte subpopulations, and the assay can be applied to samples from infants and children. In this study we describe an intracellular c

Who reads Intracellular Cytokine Expression in Whole Blood Preparations from Normals and Patients with Atopic Dermatitis?

Author

B. FERRY; P. ANTROBUS; I. HUZICKA; A. FARRELL; A. LANE; H. CHAPEL

Publisher

Oxford University Press (OUP)

Published

1997

Language

EN

Field

Immunology and Microbiology (Life Sciences)