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Can I read Genetic control of the immune response to staphylococcal nuclease: X. Isoelectric focusing spectrotypes of antinuclease antibodies detected by labeling with antigen and with anti-idiotype on EtoBox?
Genetic control of the immune response to staphylococcal nuclease: X. Isoelectric focusing spectrotypes of antinuclease antibodies detected by labeling with antigen and with anti-idiotype by Geraldine P. G. Miller; David H. Sachs is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Genetic control of the immune response to staphylococcal nuclease: X. Isoelectric focusing spectrotypes of antinuclease antibodies detected by labeling with antigen and with anti-idiotype about?
Antibody responses of inbred strains of mice to staphylococcal nuclease were studied by isoelectric focusing in polyacrylamide gels followed by in situ labeling of focused antibodies with radioactive antigen. All A/J mice examined produced antinuclease antibodies of limited heterogeneity, and although there was individual variation in the focusing patterns observed, a characteristic spectrotype produced by all of the animals could be discerned. In order to determine the possible relationship between this characteristic spectrotype and the cross-reactive idiotypes of A/J antinuclease antibodies previously described, focused antibodies were also examined with a radioactively labeled pig anti-(A/J antinuclease) anti-idiotypic antibody preparation. Using this reagent, similar spectrotypes to those observed for antigen binding were seen in all of the individual A/J sera, suggesting that cross-reactive idiotype expression is a reflection of the characteristic spectrotypes observed. The same labeled anti-idiotypic reagent revealed characteristic but different spectrotypes when used to develop focused antinuclease antibodies from individual mice of other strains, suggesting that the use of
Who reads Genetic control of the immune response to staphylococcal nuclease: X. Isoelectric focusing spectrotypes of antinuclease antibodies detected by labeling with antigen and with anti-idiotype?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Geraldine P. G. Miller; David H. Sachs
- Publisher
- Springer; Springer-Verlag; Kluwer Academic/Plenum Publishers; Springer Science and Business Media LLC (ISSN 0300-8177)
- Published
- 1984
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)