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Water self-diffusion behavior in yeast cells studied by pulsed field gradient NMR by Ki-Jeong Suh; Young-Shick Hong; Vladimir D Skirda; Vitaly I Volkov; Chung-Yung J Lee; Cherl-Ho Lee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The water self-diffusion behavior in yeast cell water suspension was investigated by pulsed field gradient NMR techniques. Three types of water were detected, which differ according to the self-diffusion coefficients: bulk water, extracellular and intracellular water. Intracellular and extracellular water self-diffusion was restricted; the sizes of restriction regions were approximately 3 and 15-20 microm, respectively. The smallest restriction size was determined as inner cell size. This size and also cell permeability varied with the growth phase of yeast cell. Cell size increased, but permeability decreased with increasing growth time. The values of cell permeabilities P(1)(d) obtained from time dependence of water self-diffusion coefficient were in good agreement with the permeabilities obtained from the exchange rate constants P(1)(eff). The values of P(1)(eff) were 7 x 10(-6), 1.2 x 10(-6) and 1.6 x 10(-6) m/s, and P(1)(d) were 6.3 x 10(-6), 8.4 x 10(-7), 1.5 x 10(-6) m/s for yeast cells incubated for 9 h (exponential growth phase), 24 h (end of exponential growth phase), and 48 h (stationary growth phase), respectively.

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Author

Ki-Jeong Suh; Young-Shick Hong; Vladimir D Skirda; Vitaly I Volkov; Chung-Yung J Lee; Cherl-Ho Lee

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-4622)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)