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Measuring the Quantity and Activity of Mitochondrial Electron Transport Chain Complexes in Tissues of Central Nervous System Using Blue Native Polyacrylamide Gel Electrophoresis by Cheolwha Jung; Cynthia M.J. Higgins; Zuoshang Xu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Measuring the Quantity and Activity of Mitochondrial Electron Transport Chain Complexes in Tissues of Central Nervous System Using Blue Native Polyacrylamide Gel Electrophoresis about?

Mitochondrial dysfunction and degeneration are associated with many neurodegenerative disorders. A dysfunctional mitochondrial electron transport chain (ETC) impairs ATP production and accelerates the generation of free radicals. To evaluate mitochondrial function, reliable methods are needed. Conventional spectrophotometric assays may not eliminate interference from nonspecific enzyme activities and do not measure quantities of specific ETC complexes. Blue native polyacrylamide gel electrophoresis (BN-PAGE) has been used to resolve mitochondrial ETC complexes. Combined with histochemical staining, it has also been applied to measure ETC enzyme activities in muscles. The current study is to determine (1) whether BN-PAGE can be used to detect ETC complexes from different regions of the central nervous system (CNS) and (2) the quantitative range of BN-PAGE in measuring the amounts and activities of different ETC complexes. By systematically varying the protein amount and the time of histochemical reactions, we have found linear ranges comparable to spectrophotometric assays for measuring enzyme activities of several ETC complexes. In addition, we found linear ranges for measuring pro

Who reads Measuring the Quantity and Activity of Mitochondrial Electron Transport Chain Complexes in Tissues of Central Nervous System Using Blue Native Polyacrylamide Gel Electrophoresis?

Author

Cheolwha Jung; Cynthia M.J. Higgins; Zuoshang Xu

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)