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Tejwani Narendra G 200812 PHD by k.cheenivijay is a document available to read on EtoBox.

The dissertation discusses the successful heterologous expression of the rat enzyme ST6Gal-I in Pichia pastoris for structural and functional studies, highlighting its role in immune regulation through the lectin CD22. The study established conditions for enzyme purification, deglycosylation, and stable isotope labeling for NMR characterization, revealing comparable enzymatic properties between glycosylated and deglycosylated forms. Initial NMR studies indicate substrate-induced conformational changes in ST

Author

k.cheenivijay

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EN