Can I read Changes to the length of the flexible linker region of the Rieske protein impair the interaction of ubiquinol with the cytochrome bc ~1~ complex on EtoBox?

Changes to the length of the flexible linker region of the Rieske protein impair the interaction of ubiquinol with the cytochrome bc ~1~ complex by Jürgen H. Nett; Carola Hunte; Bernard L. Trumpower is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Changes to the length of the flexible linker region of the Rieske protein impair the interaction of ubiquinol with the cytochrome bc ~1~ complex about?

Crystal structures of the cytochrome __bc__~1~ complex indicate that the catalytic domain of the Rieske iron–sulfur protein, which carries the [2Fe−2S] cluster, is connected to a transmembrane anchor by a flexible linker region. This flexible linker allows the catalytic domain to move between two positions, proximal to cytochrome __b__ and cytochrome __c__~1~. Addition of an alanine residue to the flexible linker region of the Rieske protein lowers the ubiquinol‐cytochrome __c__ reductase activity of the mitochondrial membranes by one half and causes the apparent __K__~m~ for ubiquinol to decrease from 9.3 to 2.6 μm. Addition of two alanine residues lowers the activity by 90% and the apparent __K__~m~ decreases to 1.9 μm. Deletion of an alanine residue lowers the activity by ≈ 40% and the apparent __K__~m~ decreases to 5.0 μm. Addition or deletion of an alanine residue also causes a pronounced decrease in efficacy of inhibition of ubiquinol‐cytochrome __c__ reductase activity by stigmatellin, which binds analogous to reaction intermediates of ubiquinol oxidation. These results indicate that the length of the flexible linker region is critical for interaction of ubiquinol with the _

Who reads Changes to the length of the flexible linker region of the Rieske protein impair the interaction of ubiquinol with the cytochrome bc ~1~ complex?

Author

Jürgen H. Nett; Carola Hunte; Bernard L. Trumpower

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)