Can I read Advancement and Obstacles in Microfluidics-based Isolation of Extracellular Vesicles on EtoBox?

Advancement and Obstacles in Microfluidics-based Isolation of Extracellular Vesicles by Megan Havers; Axel Broman; Andreas Lenshof; Thomas Laurell is a Chemistry article available to read on EtoBox.

What is Advancement and Obstacles in Microfluidics-based Isolation of Extracellular Vesicles about?

## Abstract There is a great need for techniques which enable reproducible separation of extracellular vesicles (EVs) from biofluids with high recovery, purity and throughput. The development of new techniques for isolation of EVs from minute sample volumes is instrumental in enabling EV-based biomarker profiling in large biobank cohorts and paves the way to improved diagnostic profiles in precision medicine. Recent advances in microfluidics-based devices offer a toolbox for separating EVs from small sample volumes. Microfluidic devices that have been used in EV isolation utilise different fundamental principles and rely largely on benefits of scaling laws as the biofluid processing is miniaturised to chip level. Here, we review the progress in the practicality and performance of both passive devices (such as mechanical filtering and hydrodynamic focusing) and active devices (using magnetic, electric or acoustic fields). As it stands, many microfluidic devices isolate intact EV populations at higher purities than centrifugation, precipitation or size-exclusion chromatography. However, this comes at a cost. We address challenges (in particular low throughput, clogging risks and abil

Who reads Advancement and Obstacles in Microfluidics-based Isolation of Extracellular Vesicles?

Author

Megan Havers; Axel Broman; Andreas Lenshof; Thomas Laurell

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Chemistry (Physical Sciences)