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Can I read Jianpi Yangzheng decoction suppresses gastric cancer progression via modulating the miR-448/CLDN18.2 mediated YAP/TAZ signaling on EtoBox?
Jianpi Yangzheng decoction suppresses gastric cancer progression via modulating the miR-448/CLDN18.2 mediated YAP/TAZ signaling by Xintian Xu; Yaqi Li; Ruijuan Zhang; Xu Chen; Junyu Shen; Mengyun Yuan; Yuxuan Chen; Menglin Chen; Shenlin Liu; Jian Wu; Qingmin Sun is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Jianpi Yangzheng decoction suppresses gastric cancer progression via modulating the miR-448/CLDN18.2 mediated YAP/TAZ signaling about?
Ethnopharmacological relevance Developing complementary and effective drugs with less toxicity is urgent for gastric cancer (GC) therapy. Jianpi Yangzheng Decoction (JPYZ) is a curative medical plants formula against GC in clinic while its molecular mechanism remains to be further elucidated. Aim of the study To evaluate the in vitro and in vivo anticancer efficacy of JPYZ against GC and its potential mechanisms. Materials and methods The effect of JPYZ on regulating the candidate targets were screened and examined by RNA-Seq, qRT-PCR, luciferase reporter assay, and immunoblotting. Rescue experiment was conducted to authenticate the regulation of JPYZ on the target gene. Molecular interaction, intracellular localization and function of target genes were elucidated via Co-IP and cytoplasmic-nuclear fractionation. The impact of JPYZ on the abundance of target gene in clinical specimens of GC patients was evaluated by IHC. Results JPYZ treatment suppressed the proliferation and metastasis of GC cells. RNA seq revealed JPYZ significantly downregulated miR-448. A reporter plasmid containing CLDN18 3’-UTR WT exhibited significant decrease in luciferase activity when co-transfected with m
Who reads Jianpi Yangzheng decoction suppresses gastric cancer progression via modulating the miR-448/CLDN18.2 mediated YAP/TAZ signaling?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Xintian Xu; Yaqi Li; Ruijuan Zhang; Xu Chen; Junyu Shen; Mengyun Yuan; Yuxuan Chen; Menglin Chen; Shenlin Liu; Jian Wu; Qingmin Sun
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)