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Exendin‐4 differentiation of a human pancreatic duct cell line into endocrine cells: Involvement of PDX‐1 and HNF3β transcription factors by Jie Zhou; Marco A. Pineyro; Xiaolin Wang; Maire E. Doyle; Josephine M. Egan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Exendin‐4 (EX‐4), a long acting agonist of GLP‐1, induces an endocrine phenotype in Capan‐1 cells. Under culture conditions which include serum, ∼10% of the cells contain insulin and glucagon. When exposed to EX‐4 (0.1 nM, up to 5 days), the number of cells containing insulin and glucagon increased to ∼40%. Western blot analysis detected a progressive increase in protein levels of glucokinase and GLUT2 over 3 days of EX‐4 treatment. We explored the sequence of activation of certain transcription factors known to be essential for the beta cell phenotype: PDX‐1, Beta2/NeuroD, and hepatocyte nuclear factor 3β (HNF3β). Double immunostaining showed that PDX‐1 coexisted with insulin and glucagon in EX‐4‐treated cells. Treatment caused an increase in PDX‐1 protein levels by 24 h and induced its nuclear translocation. Beta2/NeuroD protein levels also increased progressively over 24 h. HNF3β protein level increased twofold as early as 6 h after EX‐4 treatment. EMSA results indicated that EX‐4 caused a 12‐fold increase in HNF3β binding to PDX‐1 promoter area II. Beta2/NeuroD protein levels progressively increased after 24 h treatment. Differentiation to insulin‐producing cells wa

Who reads Exendin‐4 differentiation of a human pancreatic duct cell line into endocrine cells: Involvement of PDX‐1 and HNF3β transcription factors?

Author

Jie Zhou; Marco A. Pineyro; Xiaolin Wang; Maire E. Doyle; Josephine M. Egan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)