Can I read Supercritical Fluid Precipitation of Recombinant Human Immunoglobulin from Aqueous Solutions on EtoBox?

Supercritical Fluid Precipitation of Recombinant Human Immunoglobulin from Aqueous Solutions by Douglas P. Nesta; Jennifer S. Elliott; John P. Warr is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Supercritical Fluid Precipitation of Recombinant Human Immunoglobulin from Aqueous Solutions about?

Supercritical carbon dioxide was used as an antisolvent for producing recombinant human immunoglobulin G (rIgG) particulate powders. Liquid carbon dioxide (CO 2 ) was premixed with ethanol to create a single-phase, modified supercritical fluid (SCF). The modified SCF was then vigorously mixed with a pharmaceutically acceptable, aqueous formulation of rIgG, and the mixture was immediately atomized into a pressurized vessel where rapid expansion of the modified SCF extracted the aqueous phase, resulting in precipitation of the protein powder. The process was reproducible, and resulting powder products were characterized by their aqueous solubilities, and the spectroscopic profile, molecular integrity, and antigen binding activity of the individual soluble fractions. Molecular integrity was assessed via size-exclusion high-performance liquid chromatography (SEC), whereas antigen binding activity was determined using an enzyme-linked immunosorbent assay (ELISA). Attempts to characterize particle size and morphology were confounded due to the extremely deliquescent nature of the powders, causing them to absorb moisture rapidly and become gummy. Operational conditions were optimized to a

Who reads Supercritical Fluid Precipitation of Recombinant Human Immunoglobulin from Aqueous Solutions?

Author

Douglas P. Nesta; Jennifer S. Elliott; John P. Warr

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)