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What is Quantitative Hemoglobin Analysis Method about?

This document describes a calorimetric method for quantitatively determining the hemoglobin content of tissues. The method extracts hemoglobin from tissues using acetone and an acid solution, then measures the absorption of the resulting ethereal acid hematin solution at 635 nm. Tests recovered hemoglobin added to tissues and found the method reliable for lung, heart, and liver tissues. Recoveries were within 6.4% and a linear relationship was observed between hemoglobin amount and instrument readings. The

Author

waraney palit

Language

EN