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Can I read Interleukin-35 inhibits angiogenesis through T helper17/ Interleukin-17 related signaling pathways in IL-1β-stimulated SW1353 cells on EtoBox?
Interleukin-35 inhibits angiogenesis through T helper17/ Interleukin-17 related signaling pathways in IL-1β-stimulated SW1353 cells by Jie Yang; Lutian Yao; Yuxuan Li; Lin Yuan; Ruoxi Gao; Ran Huo; Hui Zhang; Liping Xia; Hui Shen; Jing Lu is a Immunology and Microbiology article available to read on EtoBox.
What is Interleukin-35 inhibits angiogenesis through T helper17/ Interleukin-17 related signaling pathways in IL-1β-stimulated SW1353 cells about?
## Background: Angiogenesis associates with chondrocytes differentiation in inflammatory arthritis. interleukin (il)- 1β stimulated sw1353 cells have a phenotype similar to this kind of chondrocytes. il-17a, a target in t helper 17 (th17)/il-17 signaling pathways, was expressed by sw1353 cells. the study aimed to explore the role of il-35 on angiogenesis in il-1β stimulated sw1353 cells and its related signaling pathways. ## Methods: Microarray dataset was downloaded from the gene expression omnibus database of arthritis cartilage. the protein-protein interaction (ppi) was analyzed for il-35, pro-angiogenic factors and the differentially expressed genes (degs). we studied the effects of il-35 on proliferation and apoptosis in il-1β stimulated sw1353 cells using cell counting kit-8 (cck-8) assay and flow cytometry. the expression of pro-angiogenic factors and il-17a were assessed by western blot and real-time pcr. added plumbagin (inhibitor of il-17a) to repeat the above experiment. the secretion of il-17a was assessed by elisa. ## Results: Il-35, pro-angiogenic factors interacted with degs to affect the function of arthritis chondrocytes. il-35 promoted il-1β-stimulated sw1353 cell
Who reads Interleukin-35 inhibits angiogenesis through T helper17/ Interleukin-17 related signaling pathways in IL-1β-stimulated SW1353 cells?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Jie Yang; Lutian Yao; Yuxuan Li; Lin Yuan; Ruoxi Gao; Ran Huo; Hui Zhang; Liping Xia; Hui Shen; Jing Lu
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)