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Nano-sized Mesoporous Hydrogen-bonded Organic Frameworks for in Situ Enzyme Immobilization by Wenping Li; Jiafu Shi; Yu Chen; Xinyue Liu; Xiangxuan Meng; Zheyuan Guo; Shihao Li; Boyu Zhang; Zhongyi Jiang is a Engineering article available to read on EtoBox.
What is Nano-sized Mesoporous Hydrogen-bonded Organic Frameworks for in Situ Enzyme Immobilization about?
Hydrogen-bonded organic frameworks (HOFs) are promising carriers for enzyme immobilization. HOFs suitable for enzyme reactions containing nicotinamide cofactors and/or larger molecule substrates are to be explored. Herein, we report the first example of nano-sized mesoporous HOFs (nmHOFs) for in situ enzyme immobilization. Taking tetrakis(4-amidiniumphenyl) methane (TAM) and 1,3,6,8-tetrakis(p-benzoic acid) pyrene (H 4 TBAPy) as building blocks, enzymes induce the assembly of TAM and H 4 TBAPy into enzyme-nmHOF (named enzyme@-TaTb) in aqueous solution. The larger π-conjugated H 4 TBAPy building block and the electrostatic/hydrogenbonding interactions between TAM in nmHOF and -COOH/-NH 2 residues in enzymes trigger the formation of TaTb with pore aperture of 2.4 nm and particle size from 19.9 ± 6.4 to 56.4 ± 20.1 nm. As a demonstration, lactate dehydrogenase (LDH) that can convert pyruvate into lactate in the presence of NADH is immobilized in TaTb nmHOF.
Who reads Nano-sized Mesoporous Hydrogen-bonded Organic Frameworks for in Situ Enzyme Immobilization?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Wenping Li; Jiafu Shi; Yu Chen; Xinyue Liu; Xiangxuan Meng; Zheyuan Guo; Shihao Li; Boyu Zhang; Zhongyi Jiang
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Engineering (Physical Sciences)