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Manufacturing of primary CAR-NK cells in an automated system for the treatment of acute myeloid leukemia by Nawid Albinger; Sabine Müller; Julia Kostyra; Jan Kuska; Sarah Mertlitz; Olaf Penack; Congcong Zhang; Nina Möker; Evelyn Ullrich is a Medicine article available to read on EtoBox.
What is Manufacturing of primary CAR-NK cells in an automated system for the treatment of acute myeloid leukemia about?
Acute myeloid leukemia (AML) still constitutes a dreadful disease with limited therapeutic options. Chimeric antigen receptor (CAR)-modified T cells struggle to target AML partly due to a lack of true AML-exclusive antigens and heterogeneity of the disease. Natural killer (NK) cells possess a high intrinsic killing capacity against AML and might be well suited for the treatment of this disease. However, the generation of primary CAR-NK cells can be difficult and time consuming. Therefore, robust systems for the generation of high numbers of CAR-NK cells under GMP conditions are required. Here we report on the automated generation of high numbers of primary CD33-targeting CAR-NK cells using the CliniMACS Prodigy^®^ platform. Automated-produced CD33-CAR-NK cells showed similar phenotype and cytotoxicity compared to small-scale-produced CD33-CAR-NK cells in vitro and were able to strongly reduce leukemic burden in an OCI-AML2 NSG-SGM3 xenograft mouse model in vivo following a cross-site shipment of the cell product. This technology might be well suited for the generation of primary CAR-modified NK cells for a broad range of targets and could facilitate clinical transition.
Who reads Manufacturing of primary CAR-NK cells in an automated system for the treatment of acute myeloid leukemia?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Nawid Albinger; Sabine Müller; Julia Kostyra; Jan Kuska; Sarah Mertlitz; Olaf Penack; Congcong Zhang; Nina Möker; Evelyn Ullrich
- Publisher
- Nature Publishing Group UK
- Published
- 2024
- Language
- EN
- Field
- Medicine (Health Sciences)