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Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones by Arenhart, Sandra; Silva, José Valter Joaquim; Flores, Eduardo Furtado; Weiblen, Rudi; Gil, Laura Helena Vega Gonzales is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones about?
The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in __Saccharomyces cerevisiae__, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were successfully recovered, resulting in viable viruses, having the kinetics of replication, focus size, and morphology similar to those of the parental virus, IBSP4ncp. In addition, the chimeric viruses remained stable for at least 10 passages in cell culture, maintaining their replication efficiency unaltered. Nucleotide sequencing revealed a few point mutations; nevertheless, the phenotype of the rescued viruses was nearly identical to that of the parental virus in all experiments. Thus, genetic stability of the chimeric clones and their phenotypic similarity to the parental virus confirm the ability of the yeast-based homologous recombination to maintain characteristics of the parental virus from which the recombinant viruses were derived. The data also support possible use of the yeast system for the manipulation of the BVDV genome.
Who reads Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Arenhart, Sandra; Silva, José Valter Joaquim; Flores, Eduardo Furtado; Weiblen, Rudi; Gil, Laura Helena Vega Gonzales
- Publisher
- SciELO; Springer-Verlag; Elsevier Editora Ltda; FapUNIFESP (SciELO); Springer Science and Business Media LLC; São Paulo SP: Sociedade Brasileira de Microbiologia (ISSN 1517-8382)
- Published
- 2016
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)