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D i s c -e l e c t r o p h o r e s i s o f E . coZi envelope p r o t e i n s on SDS a c r y l a m i d e g e l s r e p r o d u c i b l y r e v e a l e d up t o 50 d i s t i n c t p o l y p e p t i d e bands. Corresponding m o l e c u l a r w e i g h t s ranged f r o m 105,000 t o 20,000 d a l t o n s o r l e s s . M a j o r bands corresponded t o m o l e c u l a r w e i g h t s o f 73,000, 4 8 , 0 0 0 , 36,000 and 30,000 w i t h t h e l a t t e r c o n s t i t u t i n g up t o 20% o f t h e t o t a l envelope p r o t e i n depending upon t h e method o f i s o l a t i o n . Minimum l e v e l s o f d e t e c t i o n u s i n g s t a i n e d g e l s equaled 0.25 pg p r ot e i n o r 1 % o f t o t a l sample analyzed; f o r a p o l y p e p t i d e o f m o l e c u l a r w e i g h t 40,000 d a l t o n s t h i s was c a l c u l a t e d t o be e q u i v a l e n t t o 1,200 molecules p e r c e l l envelope. I n envelopes f r o m a cetBmutant s t r a i n ( r e f r a c t o r y t o c o l i c i n E2), an a d d i t i o n a l band, c o n s t i t u t i n g up t o 5% o f t h e t o t a l envelope p r o t e i n was p r e s e n t . The m o l e c u l a r w e i g h t o f t h i s p r o t e i n , w h i c h

Who reads Study of envelope proteins in E. coli cet and recA mutants by SDS acrylamide gel electrophoresis?

Author

I. B. Holland; Susan Tuckett

Publisher

Wiley (John Wiley & Sons); Alan R. Liss, Inc.; Wiley (ISSN 0091-7419)

Published

1972

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)