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High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all? by Nicolas Leulliot; Lionel Trésaugues; Michael Bremang; Isabelle Sorel; Nathalie Ulryck; Marc Graille; Ilham Aboulfath; Anne Poupon; Dominique Liger; Sophie Quevillon-Cheruel; Joël Janin; Herman van Tilbeurgh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Crystallization has long been regarded as one of the major bottlenecks in high-throughput structural determination by X-ray crystallography. Structural genomics projects have addressed this issue by using robots to set up automated crystal screens using nanodrop technology. This has moved the bottleneck from obtaining the first crystal hit to obtaining diffraction-quality crystals, as crystal optimization is a notoriously slow process that is difficult to automatize. This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples.

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Author

Nicolas Leulliot; Lionel Trésaugues; Michael Bremang; Isabelle Sorel; Nathalie Ulryck; Marc Graille; Ilham Aboulfath; Anne Poupon; Dominique Liger; Sophie Quevillon-Cheruel; Joël Janin; Herman van Tilbeurgh

Publisher

International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)