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Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans by Kornelius Zeth; Volker Schnaible; Michael Przybylski; Wolfram Welte; Kay Diederichs; Harald Engelhardt is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans about?
Omp32, the strongly anion-selective porin from Comamonas acidovorans, has been crystallized. Two crystal forms were observed, both of which belong to space group R3, but exhibit different cell dimensions a = b = 106.7, c = 140.6 A Ê (crystal form I) and a = b = 87.1, c = 135.3 A Ê (crystal form II) with one trimer per asymmetric unit. The crystals diffract to 2.2 and 2.3 A Ê resolution, respectively. Omp32 was chemically modi®ed by introducing negative charges through succinylation. The number and positions of the individual modi®cations were determined using mass spectrometry and X-ray crystallography. Chemically modi®ed porins yielded crystals of a third form, also of space group R3 but with cell constants of a = b = 109.3 and c = 263.2 A Ê (crystal form III), showing a virtually doubled c axis. Crystals of form III diffract to 3.5 A Ê resolution.
Who reads Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kornelius Zeth; Volker Schnaible; Michael Przybylski; Wolfram Welte; Kay Diederichs; Harald Engelhardt
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 1998
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)