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Intestinal Epithelial Barrier Integrity Investigated by Label-free Techniques in Ulcerative Colitis Patients by Elsie Quansah; Elena Gardey; Anuradha Ramoji; Tobias Meyer-Zedler; Bianca Goehrig; Astrid Heutelbeck; Stephanie Hoeppener; Michael Schmitt; Maximillian Waldner; Andreas Stallmach; Jürgen Popp is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Intestinal Epithelial Barrier Integrity Investigated by Label-free Techniques in Ulcerative Colitis Patients about?
## Abstract The intestinal epithelial barrier, among other compartments such as the mucosal immune system, contributes to the maintenance of intestinal homeostasis. Therefore, any disturbance within the epithelial layer could lead to intestinal permeability and promote mucosal inflammation. Considering that disintegration of the intestinal epithelial barrier is a key element in the etiology of ulcerative colitis, further assessment of barrier integrity could contribute to a better understanding of the role of epithelial barrier defects in ulcerative colitis (UC), one major form of chronic inflammatory bowel disease. Herein, we employ fast, non-destructive, and label-free non-linear methods, namely coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), two-photon excited fluorescence (TPEF), and two-photon fluorescence lifetime imaging (2P-FLIM), to assess the morpho-chemical contributions leading to the dysfunction of the epithelial barrier. For the first time, the formation of epithelial barrier gaps was directly visualized, without sophisticated data analysis procedures, by the 3D analysis of the colonic mucosa from severely inflamed UC patients. The resu
Who reads Intestinal Epithelial Barrier Integrity Investigated by Label-free Techniques in Ulcerative Colitis Patients?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Elsie Quansah; Elena Gardey; Anuradha Ramoji; Tobias Meyer-Zedler; Bianca Goehrig; Astrid Heutelbeck; Stephanie Hoeppener; Michael Schmitt; Maximillian Waldner; Andreas Stallmach; Jürgen Popp
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)